Last edited by Moll
Thursday, August 6, 2020 | History

2 edition of effect of azetidine-2-carboxylic acid on proline biosynthesis in Escherichia coli found in the catalog.

effect of azetidine-2-carboxylic acid on proline biosynthesis in Escherichia coli

Faith Irene Smith

effect of azetidine-2-carboxylic acid on proline biosynthesis in Escherichia coli

by Faith Irene Smith

  • 33 Want to read
  • 25 Currently reading

Published .
Written in English

    Subjects:
  • Plant growth inhibiting substances.,
  • Escherichia coli.

  • Edition Notes

    Statementby Faith Irene Smith.
    The Physical Object
    Pagination[8], 45 leaves, bound :
    Number of Pages45
    ID Numbers
    Open LibraryOL17908411M

    THE EFFECT OF AZETIDINE CARBOXYLIC ACID ON PROLINE BIOSYNTHESIS IN ESCHERICHIA COLI INTRODUCTION Amino acid synthesis in microorganisms is regulated by at least two distinct mechanisms: (a) end -product inhibition of one or more specific enzymes in a biosynthetic pathway and (b) re- pression of the synthesis of enzymes in the pathway. Effect of L-azetidine 2-carboxylic acid on growth and proline metabolism in Escherichia coli. Article. Nov ; Margaret M. Grant. Anne S. Brown.

    ing -GK, to have a single amino acid replacement of Asp by Asn at position , and to show a prominent increase in both-GK and -GPR activities (18). In Escherichia coli, the -GK and -GPR enzymes are believed to form a heterodimer to channel the unstable intermediate, -glutamyl phosphate, in vivo (1). As reported by Smith et al. (29), -GK. Enter search terms. Keep search filters New search. Advanced search.

    l-(−)-Azetidinecarboxylate (AC) is a toxic, natural product analog of l-proline. This study revealed the genes and biochemical strategy employed by Pseudomonas sp. strain A2C to detoxify and assimilate AC as its sole nitrogen source. The gene region from Pseudomona s sp. strain A2C required for detoxification was cloned into Escherichia coli and sequenced. Acad Acta activity addition amino acid anaerobic analysis appears arginine assembly bacterial Bacteriol bacteriophage binding Biochem Biol Biophys biosynthesis cells changes characterization Chem coli K12 complex components containing coupling cytochrome cytoplasmic dehydrogenase effect electron encoding enzyme Escherichia coli K evidence.


Share this book
You might also like
Gaming in libraries

Gaming in libraries

Police selection

Police selection

Harmonized system

Harmonized system

Hear Our Prayer with America, the Beautiful

Hear Our Prayer with America, the Beautiful

Bunny Hug

Bunny Hug

ReferNet

ReferNet

new history of Spanish literature

new history of Spanish literature

Social control, political power, and the penal question

Social control, political power, and the penal question

Communication

Communication

Nature study.

Nature study.

European labour markets

European labour markets

Walt Whitman: bard of the West.

Walt Whitman: bard of the West.

Refund of freights on frustrated voyages

Refund of freights on frustrated voyages

[ADB technical assistance pamphlet publications to the Islamic Republic of Pakistan].

[ADB technical assistance pamphlet publications to the Islamic Republic of Pakistan].

improvement of clothing through instruction at the elementary school level

improvement of clothing through instruction at the elementary school level

Effect of azetidine-2-carboxylic acid on proline biosynthesis in Escherichia coli by Faith Irene Smith Download PDF EPUB FB2

The effects of L-azetidine 2-carboxylic acid on growth and proline metabolism in a proline-requiring auxotroph of Escherichia coli are described. The homologue inhibited growth of the wild type and it, alone, did not substitute effectively for proline as a growth supplement for the by: The effects of L-azetidine 2-carboxylic acid on growth and proline metabolism in a proline-requiring auxotroph of Escherichia coli are described.

The homologue inhibited growth of the wild type and it, alone, did not substitute effectively for. Azetidinecarboxylic acid (abbreviated Aze) is a plant non-protein amino acid homologue of proline with the molecular formula C 4 H 7 NO is a heterocyclic, 4 membered ring with nitrogen as its heteroatom (an azetidine), and a carboxylic acid group substituted on one of the ring carbon atoms.

The main difference between Aze and proline is the ring of Aze has four members and the ring of Chemical formula: C₄H₇NO₂. Experientia. Nov 15;24(11) The effect of azetidinecarboxylic acid on the synthesis of proline in Escherichia coli.

Baich A, Smith by: Die Beeinflussung der Prolinsynthese in Escherichia coli durch Azetidincarbonsäure wird untersucht. The effect of azetidinecarboxylic acid on the synthesis of proline in Escherichia coli | SpringerLinkCited by: The effect of the proline analogue, L-azetidinecarboxylic acid, on proline biosynthesis in Escherichia coli has been studied.

The analogue inhibits the formation of proline precursor by acting as a false feedback inhibitor, Analysis of kinetic constants in whole-cell preparations indicate that the inhibition is noncompetitive.

Abstract. Graduation date: The effect of the proline analogue, L-azetidinecarboxylic\ud acid, on proline biosynthesis in Escherichia coli has been studied.\ud The analogue inhibits the formation of proline precursor by acting\ud as a false feedback inhibitor, Analysis of kinetic constants in\ud whole-cell preparations indicate that the inhibition is noncompetitive.\ud Comparative.

No azetidinecarboxylic acid was detected in the protein isolated from cells receiving proline together with analogue. Azetidinecarboxylic acid inhibits the growth of seedlings of mung bean (Phaseolus aumus).

The imino acid was supplied dissolved in water imbibed by. Effect of L-azetidine 2-carboxylic acid on growth and proline metabolism in Escherichia coli. Grant MM, Brown AS, Corwin LM, Troxler RF, Franzblau C. Biochim Biophys Acta, (2), 01 Oct Cited by 10 articles | PMID: Proline was among the last biosynthetic precursors to have its biosynthetic pathway unraveled.

This review recapitulates the findings on the biosynthesis and transport of proline. Glutamyl kinase (GK) catalyzes the ATP-dependent phosphorylation of L-glutamic acid.

Purification of γ-GK from Escherichia coli was facilitated by the expression of the proB and proA genes from a high-copy-number. We have evaluated the effects of the proline homologue Aze (1) (azetidinecarboxylic acid) on growth of Escherichia coli strains used to over-express recombinant forms of murine myelin basic protein (rmMBP), and on the degree of on of Aze to minimal media resulted in severe diminution of growth rate, but rmMBP could still be produced and purified.

l-Azetidinecarboxylic acid, the naturally occurring lower homologue of l-proline, inhibits chlorophyll a and phycocyanin synthesis in cells of the unicellular alga, Cyanidium inhibitory effect of the homologue on pigment synthesis in algal cells was partially reversed during subsequent incubation with proline.

Incorporation of radiolabeled proline into algal protein was. L-Azetidinecarboxylic acid is a non-protein amino acid and teratogenic agent. It is toxic in nature. Application L-Azetidinecarboxylic acid has been used as a: • collagen synthesis inhibitor • protein folding antagonist • as a standard in liquid chromatography-mass spectrometry.

Packaging 1. The presence of 2oo/zg/ml L-azetidinecarboxylic acid or cisfluoro-L-proline in Biochim. Biophys. Acta, i75 () I55 BIOSYNTHESIS OF ABNORMAL COLLAGENS. I TABLE IV INCORPORATION OF DL-[eC!-AZETIDINECARBOXYLIC ACID BY EMBRYONIC CARTILAGE Two tibiae were incubated with the labeled compounds indicated for 2 h as described in text.

proline in E. coli (14, 15). The Na+-dependent binding of proline was stereospecific, and thus the activity was inhibited by an excess amount of the L-proline analogue ~-azetidine carboxylic acid, but not by D-proline (8).

Treatment of the vesicles with the SH reagent pCMB, which inhibits proline. Azetidinecarboxylic acid (Aze) 1 is a non-protein amino acid present in sugar beets and in table beets (Beta vulgaris).

It is readily misincorporated into proteins in place of proline 2 in many. Abstract. We have evaluated the effects of the proline homologue Aze (1) (azetidinecarboxylic acid) on growth of Escherichia coli strains used to over-express recombinant forms of murine myelin basic protein (rmMBP), and on the degree of misincorporation.

l-(−)-Azetidinecarboxylate (AC) is a toxic, natural product analog of study revealed the genes and biochemical strategy employed by Pseudomonas sp. strain A2C to detoxify and assimilate AC as its sole nitrogen source. The gene region from Pseudomonas sp. strain A2C required for detoxification was cloned into Escherichia coli and sequenced.

A novel compound, 1-methylpiperidino methane sulfonate (MPMS), was found to block the osmoprotectant activity of choline and L-proline, but not glycine betaine in Escherichia coli.

MPMS was more active against salt-sensitive than salt-resistant strains, but had no effect on the salt tolerance of a mutant which was unable to transport choline. A proline analogue, azetidinecarboxylic acid, which has been reported to selectively depress collagen biosynthesis, affects lung morphogenesi vitro bsy in determining differential modifications of the rate of epithelial growth and ramification activity.

Grant MM, Brown AS, Corwin LM, Troxler RF, Franzblau C () Effect of l-azetidinecarboxylate on growth and proline metabolism in Escherichia coli. Biochim Biophys Acta – Biochim Biophys Acta –l-(−)-Azetidinecarboxylate (AC) is a toxic, natural product analog of l-proline.

This study revealed the genes and biochemical strategy employed by Pseudomonas sp. strain A2C to detoxify and.The observed sensitivity of Listeria monocytogenes to the toxic proline analogue l-azetidinecarboxylic acid (AZ) suggested that proline synthesis in Listeria may be regulated by feedback inhibition of γ-glutamyl kinase (GK), the first enzyme of the proline biosynthesis pathway, encoded by the proB gene.

Taking advantage of the Epicurian coli mutator strain XL1-Red, we performed random.